The manual provides extensive coverage of various aspects of ruminant nutrition research, including the analysis of rumen liquor for the fraction of VFA's enzymatic activity of various metabolites, the estimation of rumen fluid volume and its flow rate, and the estimation of anti-nutritional/toxic factors in various unconventional feeds using HPLC/Spectrophotometer. Additionally, the manual includes detailed analysis of milk and body condition scoring for dairy cattle, as these parameters are crucial in ruminant nutrition research. It should be noted that the manual has been designed primarily for postgraduate scholars, teachers, scientists, and feed industry personnel, and as such, the exhaustive details have been intentionally avoided.

01. Chemical Analysis 02. in vitro Rumen Studies 03. in situ Dacron Bag Studies 04. Nitrogen Fractionation By Chemical And in Vitro Methods 05. Enzymatic Methods 06. Hohenheim Gas Test - Other Applications 07. Rumen Liquor Analysis 08. Estimation Of Rumen Microbial Protein Production From Purine Derivatives in Urine 09. Rumen Fluid Volume & Its Flow Rate 10. Chromatographic Techniques And Its Application In Animal Nutrition Research 11. Fractionation Of Volatile Fatty Acids with Gas Liquid Chromatography 12. High Performance Liquid Chromatographic Analysis of Karanjin In Karanj (Honge) Seed Cake 13. Estimation of Azadirachtin In Neem Seed Cake By Hplc 14. Estimation of Ricin In Castor Bean Meal by Sds-Page 15. UV Visible Spectrophotometric Method of Estimation of Gossypol 16. Atomic Absorption Spectrophotometry. Application In Animal Nutrition Research 17. Near Infra Red Spectroscopy (Nirs) . Application In Animal Nutrition Research 18. Estimation of Antinutritional Factors In Feeds & Fodders 19. Method of Conducting Digestion Metabolism (Balance) Trial on Experimental Animals 20. Miscellaneous 21. Analysis of Milk 22. Body Condition Scoring For Dairy Cattle 23. Some Equivalents and Formulae Frequently Used In Ruminant Feed Evaluation