This book is designed to aid analysts in the analysis of plant samples. It is divided into two parts. The first part covers the various biochemical techniques used in the analysis, including the theoretical foundations and the mechanisms of the required equipment. The necessity and scope of the instruments, as well as their usefulness, are also discussed. The second part focuses on specific analytical methods, including those for sugars, amino acids, proteins, fats, enzymes, and their components, as well as nucleic acids, vitamins, antinutrients (toxicants), and minerals. Additionally, information on alkaloid and pesticide residue analysis is provided, with multiple methods given for each category. These methods include reagents, apparatus, and step-by-step procedures, with final calculations for determining the concentration of constituents included.

Part I. Analytical Techniques 1. Introduction 2. Chromatography 3. Paper Chromatography 4. Column Chromatography 5. Thin Layer Chromatography 6. Electrophoresis 7. Ion Exchange Chromatography 8. Gel-filtration Chromatography 9. Solvent Extraction 10. Dialysis, Ultra Filtration and Lyophilisation11. Centrifugation12. Gas Liquid Chromatography 13. High Pressure Liquid Chromatography14. GC/MS and LC/MS 15. Spectrophotometry 16. Infra Red Spectroscopy17. Flame Photometry 18. Atomic-absorption Spectroscopy 19. Inductively Coupled Plasma Atomic Emission Spectroscopy 20. NMR Spectroscopy 21. Radiochemical Methods 22. Mass Spectrometry 23. Polarimetry 24. Conductimetry 25. Potentiometry 26. ELISA and Radioimmunoassay (RIA) 27. Gravimetric Analysis 28. Volumetric Analysis 29. Amplification and Sequencing of Nucleic Acids.

Part II. Analytical Methods and Procedures 1. Methods of Analysis 2. Carbohydrates and their Methods of Analysis 3. Fats/Oils/Lipophilic Constituents 4. Amino Acids and Proteins 5. Enzymes 6. Nucleic Acids 7. Vitamines 8. Phenolics and Antinutrients 9. Toxins 10. Alkaloids 11. Plant Nutrients 12. Plant Pigments 13. Pesticide Residue Analysis