Animal Cell Culture: The Protocol Series: Volume 01

authored by: Ratan Kumar Choudhary, Manu M & Yashpal Singh MaliK
ISBN: 9789358879704 | Binding: Hardback | Pages: 106 | Language: English | Copyright: 2025
Length: 152 mm | Breadth: 10.72 mm | Height: 229 mm | Imprint: NIPA | Weight: 250 GMS
USD 202.00 USD 182.00
 
This book will be available from 15-Aug-2024

Animal cell culture is a common laboratory technique for maintaining live cells separated from their original tissue (primary cells) or immortal cells (cell lines). The technique of cell culture was discovered in the 19th century after the discovery of the microscope.

Presently, the culture of cells is one of the most important tools of life sciences research and is being used by investigators to understand advanced understanding  of  cell  growth  and  differentiation  and mechanisms  of  normal/ abnormal functioning of various cells. In addition to that, cell culture is employed in the generation of vaccines, the production of pharmaceutical proteins, and the safety testing of drugs.

Ratan Kumar Choudhary: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India

Manu M: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India

Yashpal Singh MaliK: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India

1  Layout, Safety Protocols and Guidelines in Cell Culture Facilities

2  Basic Equipment of Animal Cell Culture Laboratories 

3  Washing, Packing, and Sterilization of Glass and Plastic Wares 

4  Preparation of Cell Culture Media and Reagents

5  Isolation of Primary Culture of Chicken Embryo Fibroblast

6  Isolation of Primary Culture of Canine Keratinocytes

7  Isolation of Primary Culture of Canine Fibroblast 

8  Culture and Sub-culturing of Continuous Cell Lines 

9  Trypan Blue Dye Exclusion Cell Viability Assay

10  Isolation and Culture Techniques for Lymphocytes 

11  Cryopreservation Methods of Primary Cultures and Cell Lines 

12  Evaluating Cytopathic Effects of Viruses on Mammalian Cells 

13  Virus Isolation in Cell Culture System

14  One-Step Growth Curve of a Virus

15  Production of Virus in Cell Culture

16  Quantitative Analysis of Virus Titers through In-Vitro Assays 

17  Hemadsorption (HA) Assay for Virus Titration

18  Plaque Assay for Virus Detection and Quantification

19  Virus Neutralization Test

20  Techniques for Establishing and Maintaining Cell Suspension Cultures 

21  Development of Cell lines Through Virus-Mediated Techniques

22  Cell Viability and Cell Proliferation Assay

 
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