Animal Cell Culture: Volume 01 : The Protocol Series - Volume 01
authored by: Ratan Kumar Choudhary, Manu M & Yashpal Singh MaliKLength: 152 mm | Breadth: 10.72 mm | Height: 229 mm | Imprint: NIPA | Weight: 300 GMS
Keywords
animal cell culture, laboratory technique, live cells, primary cells, immortal cells, cell lines, life sciences research, cell growth, differentiation, normal functioning, abnormal functioning, vaccine generation, pharmaceutical proteins, drug safety testing, 19th century discoveryAnimal cell culture is a common laboratory technique for maintaining live cells separated from their original tissue (primary cells) or immortal cells (cell lines). The technique of cell culture was discovered in the 19th century after the discovery of the microscope.
Presently, the culture of cells is one of the most important tools of life sciences research and is being used by investigators to understand advanced understanding of cell growth and differentiation and mechanisms of normal/ abnormal functioning of various cells. In addition to that, cell culture is employed in the generation of vaccines, the production of pharmaceutical proteins, and the safety testing of drugs.
Ratan Kumar Choudhary: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India
Manu M: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India
Yashpal Singh MaliK: College of Animal Biotechnology Guru Angad Dev Veterinary and Animal Sciences Univeristy, Ludhiana, Punjab, India
1 Layout, Safety Protocols and Guidelines in Cell Culture Facilities
2 Basic Equipment of Animal Cell Culture Laboratories
3 Washing, Packing, and Sterilization of Glass and Plastic Wares
4 Preparation of Cell Culture Media and Reagents
5 Isolation of Primary Culture of Chicken Embryo Fibroblast
6 Isolation of Primary Culture of Canine Keratinocytes
7 Isolation of Primary Culture of Canine Fibroblast
8 Culture and Sub-culturing of Continuous Cell Lines
9 Trypan Blue Dye Exclusion Cell Viability Assay
10 Isolation and Culture Techniques for Lymphocytes
11 Cryopreservation Methods of Primary Cultures and Cell Lines
12 Evaluating Cytopathic Effects of Viruses on Mammalian Cells
13 Virus Isolation in Cell Culture System
14 One-Step Growth Curve of a Virus
15 Production of Virus in Cell Culture
16 Quantitative Analysis of Virus Titers through In-Vitro Assays
17 Hemadsorption (HA) Assay for Virus Titration
18 Plaque Assay for Virus Detection and Quantification
19 Virus Neutralization Test
20 Techniques for Establishing and Maintaining Cell Suspension Cultures
21 Development of Cell lines Through Virus-Mediated Techniques
22 Cell Viability and Cell Proliferation Assay