The book is written in a very simple and lucid manner so that everybody can read and understand it very easily. The book is useful for scientists, teachers, students, officers, diagnosticians and laboratory technicians as cell culture has become an essential and indispensable tool in many branches of life sciences and application of cell culture is getting increased exponentially day by day in various fields of biological and medical research arena. This book will provide detailed information on all the aspects of the cell culture starting from establishment of a cell culture laboratory, primary culture, secondary culture, media filtration, collection, preservation and dispatch of samples for diagnosis of viral diseases, cell line authentication and characterization, contamination and curing, cryopreservation of cells and revival of cells besides description on ELISA, SNT, virus titration etc. In my opinion, this book will be extremely useful to the persons who are directly and indirectly involved in cell culture work for various biological experiments. Finally, students and examinees can enrich their knowledge on cell culture from the book and can face any challenge easily and confidently. s on the latest developments on biotechnological approaches for fish disease diagnostic, infection and immunity of brood carps, cryoconservation of fishes, probiotics and nanotechnology in aquaculture are of paramount interest, in addition to information on prawn aquaculture, ornamental fish farming and trade. Information on various software and their application for exploratory data analysis and data mining leading to knowledge discovery and visualization is the main attraction of the book. Another important feature of the book is that one can find appropriate as well as illustrated examples exclusively with fisheries data. The statistics section includes biometrical and qualitative techniques in genetics and selective breeding of fish, besides fundamental statistical test, design of experiments and sampling methods for planning of experiments and survey in fisheries and aquaculture research. The book also includes econometric approach for technical efficiency estimation and input-output analysis, project evaluation, and impact assessment, linear programming, market intelligence, fisheries legislation, policy and IPR issues all of which are new in the field of fisheries and aquaculture.
01. Introduction 02. Principles of Cell Culture 03. The Cell and its Environment 04. Types of Cells And Cell Culture 05. Cell Culture: Applications, Advantages and Disadvantages 06. Ideal Cell Culture Laboratory with Equipments 07. Growth and Maintenance of Cells in Culture 08. Components of Medium and its Preparation 09. Primary Cell Culture 10. Maintenance of Cell Culture 11. Packaging and Transportation of Cells 12. Description of Commonly Used Cell Lines 13. Subculturing of Cells in Different Vessels 14. Scaling Up of Animal Cell Culture 15. Cell Line Authentication and Characterization 16. Cryopreservation and Thawing/Revival of Cells 17. Contamination of Cell Culture and Curing 18. Counting of Cells 19. Isolation and Identification of Virus in Cell Culture 20. Virus Assays 21. Collection, Preservation and Transport of Various 22. Specimens for Laboratory Diagnosis 23. Collection of Materials for Diagnosis of Viral 24. Diseases 25. Processing of Laboratory Specimens, Inoculation into Laboratory Animals and Embryonated Eggs for Pathogen Isolation 26. Serum Neutralization Test 27. Enzyme Linked munosorbent Assay (ELISA) 28. Biosafety Measures and Risk Management
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