Analysis of rumen liquor for fraction of VFA’s enzymatic activity of various metabolites and estimation of rumen fluid volume and its flow rate are covered in depth. It was followed by estimation of anti-nutritional / toxic factors in various un-conventional feeds using HPLC / Spectrophotometer, detail analysis of milk and body condition scoring for dairy cattle are included as assessment of these parameters are important in Ruminant Nutrition Research.

Necessary practical work is included; the exhaustive details have been avoided, since the manual is primarily meant for postgraduate scholars, teachers, scientists and feed industry personnel use.

T.M. Prabhu M.V.Sc., Ph.D.,Associate Professor and Head,Department of Animal Nutrition, Veterinary College, KVAFSU, Hassan-573 201, Karnataka,India

K.Chandrapal Singh M.V.Sc., MS (Canada), Ph.D.,Professor and Head,Department of Animal Nutrition, Veterinary College, KVAFSU, Bengaluru-560 024, Karnataka, India

01. Chemical Analysis 02. in vitro Rumen Studies 03. in situ Dacron Bag Studies 04. Nitrogen Fractionation By Chemical And in Vitro Methods 05. Enzymatic Methods 06. Hohenheim Gas Test - Other Applications 07. Rumen Liquor Analysis 08. Estimation Of Rumen Microbial Protein Production From Purine Derivatives in Urine 09. Rumen Fluid Volume & Its Flow Rate 10. Chromatographic Techniques And Its Application In Animal Nutrition Research 11. Fractionation Of Volatile Fatty Acids with Gas Liquid Chromatography 12. High Performance Liquid Chromatographic Analysis of Karanjin In Karanj (Honge) Seed Cake 13. Estimation of Azadirachtin In Neem Seed Cake By Hplc 14. Estimation of Ricin In Castor Bean Meal by Sds-Page 15. UV Visible Spectrophotometric Method of Estimation of Gossypol 16. Atomic Absorption Spectrophotometry. Application In Animal Nutrition Research 17. Near Infra Red Spectroscopy (Nirs) . Application In Animal Nutrition Research 18. Estimation of Antinutritional Factors In Feeds & Fodders 19. Method of Conducting Digestion Metabolism (Balance) Trial on Experimental Animals 20. Miscellaneous 21. Analysis of Milk 22. Body Condition Scoring For Dairy Cattle 23. Some Equivalents and Formulae Frequently Used In Ruminant Feed Evaluation