This book, "Laboratory Manual of Biochemistry: Methods and Techniques," represents the culmination of 17 years of teaching and research experience on the part of its authors. As a relatively recent scientific discipline, biochemistry has gained prominence due to the breadth and rapid pace of its research, which encompasses a broad range of other disciplines. Because biochemistry operates at the molecular level, it has become indispensable to the study of biological phenomena. This book provides a comprehensive compilation of frequently utilized techniques in a single volume, aimed at undergraduate and postgraduate students in fields such as biochemistry, biotechnology, microbiology, agricultural sciences, environmental science, botany, zoology, nutrition, pharmaceutical science, and other related subjects.

The first edition of this book is divided into 11 Units, each comprising the principle, requirements, procedure, calculation, and observations for a specific experiment. At the end of each Unit, additional reading materials are provided for further exploration. The book also includes a notes section that provides important precautions, warnings, and tips for each experiment. Additionally, there are 12 appendices that offer detailed information on topics such as basic chemistry, buffer preparations, and other essential aspects of experimentation.

This book will serve as a valuable resource for researchers, as it encompasses classical biochemistry techniques as well as modern PCR methods. It will help streamline the research process by providing researchers with the necessary protocols in a single, accessible source.

1. General laboratory principles. 2. Carbohydrates. 3. preparations and other aspects required for the conduct of the experiments. 4. Lipids. 5. Nucleic acids. 6. Enzymes. 7. Vitamins. 8. Pigments. 9. Phenolics. 10. Anti nutritional factors. 11. Plant hormones.