The book "Principle of Biotechnology and Genetics" has been designed for both undergraduate and postgraduate students. It covers the major practical aspects of the subject and adheres to the prescribed syllabus for Indian universities. The language used is simple and easy to understand, presented in a point-wise format for better comprehension. The book consists of 22 chapters that detail the significant achievements in biotechnology, bioinstrumentation, supporting methodology, tissue culture techniques, genetic engineering techniques, and their applications. Additionally, the book includes a glossary of technical terms, abbreviations, unit details, and references for added convenience. This book will be immensely helpful for both teachers and students in the field of biotechnology.

Part I – Introduction: 1. Introduction of Biotechnology, Part II - Landmark Achievements in Biotechnology: 2. Landmark Achievements in Plant Tissue Culture and, Genetic Engineering, Part III - Supporting Methodologies: 3. Requirements for Plant Tissue Culture Laboratory, 4. Techniques in Plant Tissue Culture, 5. Media Components and Preparations, 6. Sterilization Techniques and Inoculation of Various Explants, 7. Aseptic Manipulation of Various Explants, Part IV - Propagation Techniques: 8. Callus Induction, 9. Micropropagation, 10. Micropropagation of Important Crops, 11. Anther, Embryo and Endosperm Culture, 12. Hardening/Acclimatization of Regenerated Plantlets, 13. Somatic Embryogenesis and Synthetic Seed Production 14. Isolation and Demonstration of Protoplast, Part V - Genetic Engineering Techniques: 15. Demonstration of Isolation of DNA, 16. Demonstration of Gene Transfer Techniques, Direct Methods, 17. Demonstration of Gene Transfer Techniques, Indirect Methods, 18. Demonstration of Conformation of Genetic Transformation, 19. Blotting Techniques 20. Demonstration of Gel Electrophoresis Techniques, Part VI- Special Topics: 21. Bio-instrumentation 22. Biotechnological Achievements