This laboratory manual has been developed for students to facilitate comprehension of fundamental plant pathological laboratory techniques related to the isolation of pathogens. The manual covers the preservation of disease samples, the demonstration of Koch's postulates, the examination of various classes of fungicides and antibiotics, as well as methods for preparing and applying these agents. The manual also includes bioassays and biocontrol methods for plant pathogens, as well as the identification of important fungal pathogens. The manual is a fully-colored book that features digitized images to aid in the identification of diseases and pathogens. It also includes explanations of new terminologies to enhance students' understanding of the subject. This book will be valuable to novices, students, instructors, scientists, and researchers working in the fields of Plant Pathology and Agricultural Microbiology.

1. Acquaintance with different plant pathological appliances in the laboratory 2. Preparation of culture media for fungi and bacteria 3. Isolation techniques 4. preservation of disease samples 5. Demonstration of Koch’s postulates 6. Study of different groups of fungicides and antibiotics 7. Preparation of fungicides (Bordeaux mixture, Bordeaux paste, Chestnut compound) 8. Methods of application of fungicides (seed, soil and foliar) 9. Bio-assay of fungicides (poisoned food technique, inhibition zone technique and slide germination technique) 10. Bio control of plant pathogens (dual culture technique, seed treatment) 11. Study of Pythium, Phytophthora and Albugo 12. Study of Sclerospora, Peronosclerospora, Pseudoperonospora, Peronospora, Plasmopara and Bremia 13. Study of genera Mucor and Rhizopus 14. Study of Oidium, Oidiopsis, Ovulariopsis, Erysiphe, Phyllactinia, Uncinula and Podosphaera 15. Study of Puccinia (different stages), Uromyces, Hemileia 16. Study of Sphacelotheca, Ustilago and Tolyposporium 17. Study of Agaricus, Pleurotus and Ganoderma 18. Study of Septoria, Colletotrichum, Pestalotiopsis and Pyricularia 19. Study of Aspergillus, Penicillium,Trichoderma, and Fusarium 20. Study of Helminthosporium, Drechslera, Alternaria, Stemphylium, Cercospora, Phaeoisariopsis, Rhizoctonia and Sclerotium