Techniques in Molecular Diagnostics

authored by: Kandarpa Kumar Saikia
ISBN: 9789385516528 | Binding: Hardback | Pages: 142 | Language: English | Copyright: 2016
Length: 152 mm | Breadth: 13.3 mm | Height: 229 mm | Imprint: NIPA | Weight: 400 GMS
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The text discusses a book that provides an in-depth exploration of molecular biology and diagnostics, starting from basic concepts to advanced assays offered by diagnostic laboratories. It includes detailed information on establishing a laboratory and laboratory mathematics, which is useful for preparing common laboratory solutions. The book features protocols for nucleic acid extraction from various sources, which can be easily applied to isolate high-quality DNA and RNA from human blood and tissues. It also presents a range of molecular diagnostic tools that are easy to understand and can be used for cancer diagnosis. The book includes examples of biomarker detection protocols for specific cancer types using molecular diagnostic tools, which are highly sensitive, cost-effective, and can provide faster and accurate diagnosis. Finally, the book covers quality assurance and quality control strategies for molecular diagnostic laboratories, emphasizing the design of quality manuals for these laboratories.

Kandarpa Kr. Saikia, Ph. D. Assistant Professor, Department of Bioengineering, Gauhati University, Guwahati, Assam, India

1. Introduction to Molecular Diagnostics by Partha Pratim Sarma 2. General Calculation for Making Solutions in Molecular Diagnostic Laboratory by Pranjal Kr. Baruah 3. Basic Requirements for Setting-up a Molecular Diagnostic Laboratory by Kandarpa Kr. Saikia 4. Nucleic Acid Extraction Protocols by Partha Pratim Sarma and Kandarpa Kr. Saikia 5. Protocol to Perform Agarose Gel Electrophoresis by Sukanta Nath and Partha Pratim Sarma 6. Protocol for Qualitative PCR Using Genomic DNA or cdna as Template by Kandarpa Kr. Saikia and Sukanta Nath 7. DNA Sequence Analysis by Kandarpa Kr. Saikia and Vedant Vikrom Borah 8. Protocol for Synthesis of cDNA from Total RNA by Sukanta Nath and Partha Pratim Sarma 9. Protocol to Perform RT-PCR (Reverse Transcriptase PCR for Gene Expression Studies) by Kandarpa Kr. Saikia 10. Protocol to Perform Quantitative Real Time PCR (qpcr) by Partha Pratim Sarma and Sukanta Nath 11. Protocol to Perform FISH by Manoj Kr. Panigrahi 12. Examples of Biomarker Detection Protocols Using Molecular Diagnostic Tools -Acute Myeloid Leukaemia, Glioma and Chronic Myeloid Leukaemia Molecular Markers by Kandarpa Kr. Saikia, Partha Pratim Sarma, Sukanta Nath and Dushyant Kumar 13. Quality Assurance and Quality Control in Molecular Diagnostics by Rupjyoti Kalita and Kandarpa Kr. Saikia

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