This book covers a comprehensive range of topics, with a particular focus on the latest information regarding mesenchymal stem cells (MSCs). It is divided into 17 chapters, which explore various aspects of stem cell technology, such as the different types of stem cells and their isolation from different animal sources. The book also includes information about the culture and characteristics of MSCs, as well as their osteoinductive potential.

In addition, the book provides protocols for the isolation of MSCs from bone marrow, fat tissue, and embryonic stem cells. It also discusses the application of bioengineered stem cell technologies in skin wound healing, burn wound healing, critical bone defect healing, large bone defect healing, corneal wound healing, and liver regeneration.

The book is intended to serve as a valuable resource for undergraduate and postgraduate research scholars, teachers, biologists, and clinicians involved in animal treatment and research. It features over 150 high-quality color photographs illustrating various aspects of stem cell isolation, culture, morphology, characteristics, and application in surgical interventions.

1. Stem Cells: Definition and Its Application, 2. Isolation, Proliferation and Morphological Characteristics of Bone-marrow Derived Mesenchymal Stem Cells  (BM-MSC) from Different Animal Species, 3. Culture, Characterization and Differentiation Potential of Rat Bone Marrow Derived Mesenchymal Stem Cells, 4.  Isolation, Proliferation, Characterization of Rabbit Bone-Marrow Derived Mesenchymal Stem Cells (rBMSC), 5. In vitro Osteoinduction Potential of A Novel Silica Coated Hydroxyapatite Bioscaffold Seeded with Rabbit Mesenchymal Stem Cell, 6. Implantation of MSC-Ceramic Construct for Repair of Critical Sized Bone Defect in Rabbit Model, 7. Mesenchymal Stem Cells Seeded Bioceramic Construct for Bone Regeneration in Large Critical-size Bone Defect in Rabbit, 8.   Effect of Medical Grade Chitosan Powder with Mesenchymal Stem Cell for Full Thickness Skin Wound Healing in Rat Model, 9.  Evaluation of Mesenchymal Stem Cell Seeded Decellularized Porcine Intestine and Decellularized Cornea for the Repair of Corneal Defect in Rabbit Model, 10. Effect of PDGF-beta Gene Activated Acellular Matrix and Stem Cell Transplantation on Full Thickness Burn Wound in Rat Model, 11. Evaluation of Healing Potential of Chitosan and Porcine Small Intestinal Sub-Mucosa with Mesenchymal Stem Cells and Murine EGF for Full Thickness Burn Wound in Rat Model, 12.Evaluation of Mesenchymal Stem Cells with Conditioned Media and m-EGF for Regeneration of Liver Tissue after Partial Hepatectomy in Rats, 13. Role of Embryonic Stem cell (ESC)-Hydroxyapatite Construct with Growth Proteins for Osteogenesis in the Repair of Bone Defects in Rabbit Model, 14. Protocol for Bone-marrow Derived MSC Isolation from Rat, 15. Protocol for Fat Derived MSC Isolation from Rat, 16. Protocol for Bone Marrow Derived MSC Isolation from Rabbit, Dog, Goat, Sheep and Pig, 17. Protocol for Mesenchymal Stem cell Passaging References & Further Reading, Colour Plates