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Molecular Diagnostics : A Practical Manual

Birg (Dr) B.K. Khanna, Nina Khanna

  • Country of Origin India
  • ImprintNIPA
  • ISBN9789389571806
  • BindingHardback
  • Year of Publishing2020
  • Number Of Pages118 pages, 25
  • LanguageEnglish
  • Available Formats

     
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      USD 66.99
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  • About The Author
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  • This practical manual on molecular diagnostics is written to help the students and faculties who are doing practical of molecular biology, biochemistry and biotechnology. This practical manual covers the syllabus of ICAR as per 5th dean committee for B.Sc. This practical manual can also be used by the students of under graduate and post graduate of other streams of biological sciences. This manual explains step wise laboratory practical procedure for different experiments. This manual will also be helpful for those students and researchers who want to plan the experiment in molecular biology laboratory.

    The authors are also engaged in research and teaching in this area and having wide experience in molecular biology. In the end of each experiment some questions have been asked to solve by the students that will help in better understanding of the topic. This manual also contains set of objective questions related to the molecular diagnostics that will be very useful for their competitive exams and regular theoretical exams as well.

  • Diwakar Singh, Assistant Professor (Biochemistry), Department of Plant Molecular Biology and Biotechnology,, ASPEE College of Horticulture and Forestry,, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – India

    K.P. Suthar, Assistant Professor, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – India

    Reetu Mehta, Lecturer, Naranlala College of Professional and Applied Sciences, Navsari-396450, Gujarat, India

  • No eChapter available for this eBook
  • Diagnosis of Drought Tolerance in Crop Plants by PEG Method

    Identification of Salt Tolerant Crop Variety by Glycine Betaine (GB) Method

    Total Protein Estimation by Folin-Lowry’s Method

    Protein Electrophoresis Using SDS-PAGE

    Enzyme Extraction and Assay Study of Polyphenol Oxidase (PPO; EC 1.14.18.1)

    Enzyme Extraction and Assay Study of Catalase (EC 1.11.1.6)

    Enzyme Extraction and Assay Study of Guaiacol Peroxidase (POX) (EC 1.11.1.7)

    Enzyme Extraction and Activity Study of Superoxide Dismutase (EC 1.15.1.1)

    Peroxidase Isozyme Study by Electrophoresis Method

    DNA Isolation from Plant Sample and Quantification by Spectrophotometer

    Quality Check of Plant Genomic DNA on Agarose Gel Electrophoresis

    Randomly Amplified Polymorphic DNA (RAPD) and Electrophoresis

    Southern Blotting Experiment

    Total RNA Isolation from Plants and its Quantification by Spectrophotometer

    Quality Check of Total RNA on Agarose Gel

    cDNA Synthesis from Isolated Plant Total RNA

    Indirect Competitive ELISA

    Objective Questions

     

     

  • c5hpv2

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