This manual, designed for undergraduate and postgraduate students in the field of biological sciences, provides step-by-step instructions for conducting various experiments in a molecular biology laboratory. In addition to being useful for students and researchers planning experiments in this field, the manual is also beneficial for those who wish to enhance their understanding of molecular diagnostics. The authors, who have extensive experience in molecular biology and are actively involved in research and teaching in the field, have included a set of objective questions at the end of each experiment to aid in comprehension. This manual will also be helpful for students preparing for competitive exams or regular theoretical exams.

Diwakar Singh: Assistant Professor (Biochemistry), Department of Plant Molecular Biology and Biotechnology,, ASPEE College of Horticulture and Forestry,, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – India

K.P. Suthar: Assistant Professor, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – India

Reetu Mehta: Lecturer, Naranlala College of Professional and applied Sciences, Navsari-396450, Gujarat, India

Diagnosis of Drought Tolerance in Crop Plants by PEG Method

Identification of Salt Tolerant Crop Variety by Glycine Betaine (GB) Method

Total Protein Estimation by Folin-Lowry’s Method

Protein Electrophoresis Using SDS-PAGE

Enzyme Extraction and Assay Study of Polyphenol Oxidase (PPO; EC 1.14.18.1)

Enzyme Extraction and Assay Study of Catalase (EC 1.11.1.6)

Enzyme Extraction and Assay Study of Guaiacol Peroxidase (POX) (EC 1.11.1.7)

Enzyme Extraction and Activity Study of Superoxide Dismutase (EC 1.15.1.1)

Peroxidase Isozyme Study by Electrophoresis Method

DNA Isolation from Plant Sample and Quantification by Spectrophotometer

Quality Check of Plant Genomic DNA on Agarose Gel Electrophoresis

Randomly Amplified Polymorphic DNA (RAPD) and Electrophoresis

Southern Blotting Experiment

Total RNA Isolation from Plants and its Quantification by Spectrophotometer

Quality Check of Total RNA on Agarose Gel

cDNA Synthesis from Isolated Plant Total RNA

Indirect Competitive ELISA

Objective Questions