This practical manual can also be used by the students of under graduate and post graduate of other streams of biological sciences. This manual explains step wise laboratory practical procedure for different experiments. This manual will also be helpful for those students and researchers who want to plan the experiment in molecular biology laboratory.

The authors are also engaged in research and teaching in this area and having wide experience in molecular biology. In the end of each experiment some questions have been asked to solve by the students that will help in better understanding of the topic. This manual also contains set of objective questions related to the molecular diagnostics that will be very useful for their competitive exams and regular theoretical exams as well.

Diwakar Singh: Assistant Professor (Biochemistry), Department of Plant Molecular Biology and Biotechnology,, ASPEE College of Horticulture and Forestry,, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – India

K.P. Suthar: Assistant Professor, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Eru Char Rasta, Dandi Road, Navsari - 396 450, Gujarat – India

Reetu Mehta: Lecturer, Naranlala College of Professional and applied Sciences, Navsari-396450, Gujarat, India

Diagnosis of Drought Tolerance in Crop Plants by PEG Method

Identification of Salt Tolerant Crop Variety by Glycine Betaine (GB) Method

Total Protein Estimation by Folin-Lowry’s Method

Protein Electrophoresis Using SDS-PAGE

Enzyme Extraction and Assay Study of Polyphenol Oxidase (PPO; EC 1.14.18.1)

Enzyme Extraction and Assay Study of Catalase (EC 1.11.1.6)

Enzyme Extraction and Assay Study of Guaiacol Peroxidase (POX) (EC 1.11.1.7)

Enzyme Extraction and Activity Study of Superoxide Dismutase (EC 1.15.1.1)

Peroxidase Isozyme Study by Electrophoresis Method

DNA Isolation from Plant Sample and Quantification by Spectrophotometer

Quality Check of Plant Genomic DNA on Agarose Gel Electrophoresis

Randomly Amplified Polymorphic DNA (RAPD) and Electrophoresis

Southern Blotting Experiment

Total RNA Isolation from Plants and its Quantification by Spectrophotometer

Quality Check of Total RNA on Agarose Gel

cDNA Synthesis from Isolated Plant Total RNA

Indirect Competitive ELISA

Objective Questions