The book presents various essential techniques of recombinant DNA technology, such as the isolation and transformation of bacteria, the isolation and characterization of nucleic acids, the construction and screening of gene libraries, DNA sequencing, RFLP and RAPD gene mapping, genetic transformation, and molecular analysis of transgenic plants. Additionally, the book provides protocols for important techniques currently used in plant biotechnology. It also includes methods for RFPL and RAPD analysis, as well as a practical example of an actual research problem in the field of plant genetic engineering. The text is complemented by a glossary of important terms and the appendix provides information on the preparation of common buffers and reagents used in the various protocols.

1. Background - Plant Biotechnology, 2. Isolation, Restriction and Agarose Gel Electrophoresis of E.coli Plasmid DNA, 3. Transformation of Bacterial Cells , 4. Preparation of Bacterophage? DNA ,5. Isolation and Purification of Genomic DNA from Plants, 6. Isolation of Total RNA and in vitro Translation of Poly (A)+RNA, 7. Isolation of and Analysis of Polyribosomes and Ribosomal Proteins,8. Preparation of a Probe: Radioactive Labeling of Nucleic Acids, 9. Southern and Northern Blotting and Hybridization, 10. Construction of Genomic Library, 11. Screening of Genomic Library, 12. Construction and Screening of cDNA Library, 13. PCR and RT-PCR Methods, 14. M13 Cloning for Di-deoxy DNA Squencing, 15. DNA Sequencing Methods, 16. Isolation and in vitro Culture of Protoplasts, 17. Methods of Genetic Transformation of Plants, 18. Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD) Analysis, 19. Development and Characterization of Transgenic Crop Plants