Keywords
veterinary diagnostic fluids, livestock blood analysis, dairy cattle milk health, non-invasive animal diagnostics, fine needle aspirate veterinary, animal health monitoring, livestock disease detection, blood tests for livestock, urine analysis in animals, saliva diagnostics in animals, livestock metabolic disorder testing, immune status in livestock, mastitis detection in dairy cows, milk somatic cell count, FNA biopsy in animals, veterinary urine testing, animal saliva hormone analysis, non-invasive veterinary testing, pathological changes in animals, biological fluid testing livestock, animal kidney function tests, early disease indicators livestock, hormonal changes in animals, veterinary metabolic testing, disease indicators in animal urine, animal stress markers saliva, animal cancer detection saliva, animal physiological assessment, livestock health diagnosticsTechniques in Veterinary Cytology: Volume 07 : The Protocol Series - Volume 07
authored by: Ratan Kumar Choudhary, Yashpal Singh Malik , Devendra Pathak, Shanti ChoudharyLength: 152 mm | Breadth: 9.09 mm | Height: 229 mm | Imprint: NIPA | Weight: 310 GMS
Biological fluids such as blood, milk, fine needle aspirates, urine, and saliva from livestock play a critical role in veterinary medicine, animal husbandry, and agricultural research. These fluids serve as non-invasive or minimally invasive sources of valuable diagnostic information, enabling the monitoring of animal health, disease detection, and the assessment of physiological and metabolic states. Blood, the most widely studied fluid, provides insights into an animal's overall health, immune status, and the presence of infections or metabolic disorders. Milk, particularly in dairy cattle, is crucial for assessing mammary gland health and detecting conditions like mastitis. Detection of milk somatic cells on a routine basis and keeping them low is desirable for milk and milk product quality. Fine needle aspirates (FNA) allow for the examination of specific tissues, such as lymph nodes and cancer masses, offering detailed information on cellular composition and the presence of pathological changes in cell morphology and cellular composition. Urine and saliva are increasingly recognized for their diagnostic potential. Urine analysis can reveal metabolic and kidney function and provide early indicators of systemic diseases. Saliva, being easy to collect, is a growing focus for non-invasive monitoring, hormonal changes, cancer, diseases and stress.
Section A. Collection of Biological Fluids
1. Blood Collection from Bovine and Canine
2. Milk Collection from Cow
3. Collection of Fine Needle Aspirates (FNA) from Canine Mammary Tumors
4. Collection of Urine from Animals Including Rodents
5. Collection of Saliva from Dogs
Section B. Blood
6. Isolation of Peripheral Blood Mononuclear Cells
7. Preparation of PBMC Smear
8. Immunostaining of PBMC For Macrophage Identification
Section C. Milk
9. Isolation of Milk Somatic Cells
10. Staining of Milk Somatic Cells
11. Immunostaining of Milk Somatic Cells
Section D. Fine Needle Aspirates
12. Preparation of FNA Smear
13. Staining of FNA Smears- Giemsa Staining
14. Staining of FNA Smears- Leishman Staining
15. Immunostaining of FNA Smears for The Identification of Proliferating Cells
Section E. Analysis of Morphology of Cells
16. Introduction to Imagej
17. Cell Counting Using Imagej
18. Cytomorphological Analysis of Cells