This book brings together practical, step-by-step protocols that cover the full spectrum of veterinary anatomy and allied laboratory techniques. Part I focuses on gross specimen work—embalming of animals, bone collection and preparation for osteology, museum curation (including taxidermy and alternative teaching tools), glycerin preservation of large specimens, and embryonic/foetal age estimation for developmental studies. Part II presents core histological methods from fixation and paraffin embedding to microtomy and routine/special staining. Detailed chapters address tissue-specific needs (bone, eye, endocrine organs), classic stains (H&E) and targeted protocols for mucopolysaccharides, amyloid, pigments, lipids, nerves, and the diffuse neuroendocrine system, alongside cytology, cytochemistry and cytogenetics. Part III introduces advanced and special techniques including enzyme and lectin histochemistry, immunocytochemistry for stem cells, electron microscopy (SEM/TEM), confocal microscopy, and entry-level molecular analysis (PCR, PCR-RFLP), capped with a concise guide to commonly used special stains and necropsy examination.

Designed for undergraduates, scholars, anatomy and pathology instructors, and laboratory personnel, the book emphasizes reproducible results, specimen integrity, and pedagogical utility in teaching museums.

The protocol-driven coverage, authored by subject specialists, supports ICAR-aligned practical training and day-to-day lab workflows—from gross preparation to ultrastructural and molecular visualization—making it a single, dependable bench reference for veterinary anatomy and histology.

Pawan Kumar, Professor, Department of Veterinary Anatomy, Lala Lajpat Rai Univerity of Veterinary and Animal Sciences, Hissar, Harayana, India

1. Embalming of Animals - P.V.S. Kishore

2. Collection And Preparation of Bones for Osteological Study Purposes - Archana Pathak, M.M. Farooqui and Ajay Prakash

3. Biomechanical Techniques in Veterinary Science - Subhash C. Dubal and D.M. Bhayani

4. Biometry and Micrometry Practices in Veterinary Anatomy - Kamal Sarma

5. Staining Procedures for Whole Brain and its Sections - S.P. Ingole

6. Taxidermy: The Life After Death - Santosh A. Gaikwad

7. An Alternative Method of Specimen Preparation-Anatomical Teaching Tools - R. Menaka and S. Chaurasia

8. Protocol for Preservation of Gross Specimens by Glycerin Method - M.H. Girish, K. Manjunatha, R.V.  Prasad and K.V. Jamuna

9. How to Develop an Anatomy Museum - Rakhi Vaish, Payal Jain and Nidhi Gupta

10. Estimation of Embryonic/Foetal Age for Developmental Anatomy Research - Prabhakar Kumar and M.M. Farooqui

Histological Techniques

11. Routine Paraffin Wax Embedding Technique - P.C. Kalita and Arup Kalita

12. Sectioning for Light-Microscopy (Paraffin Blocks) - S. Usha Kumary

13. Procedures for Bone Tissue - Paramasivan, S. and Sivagnanam, S.

14. Hematoxylin and Eosin Staining Techniques - Rajesh Rajput and Virender Pathak

15. Fixation of Eye Ball and Special Staining - Rakesh Kumar Baraihya and Pawan Kumar

16. Staining Protocols for Connective Tissue Fibers - Balwant Meshram

17. Histochemical Techniques for Mucopolysaccharides - Pawan Kumar and Varinder Uppal

18. Histological Staining Techniques for Amyloid - Sanjay Kumar Bharti and Avnish Kumar Gautam

19. Methods for Lipids/Fat - Vishnudeo Kumar, Anil Sharma, K.N. Vyas and Y.L. Vyas

20. Techniques for Nerve Cells and Fibers- Partha Das and Pawan Kumar

21. Demonstration of the Diffuse Neuroendocrine System - U.K. Mishra

22. Staining Methods for Pigments - Ganga Naik, S. and Pawan Kumar

23. Miscellaneous Histological Techniques - Pawan Kumar, Shailja Bansal and Shikha Saini

24. Histological Techniques for Endocrine Organs - Massarat Khan, Andleeb Rafiq and Nuzhat Nasar

25. Cytological, Cytochemical and Cytogenymic Staining Methods for Blood Cells - Suresh Mehta and Ishwar Singh

26. Natural Alternatives to Histological Staining Techniques - Amit Mitra and R. Partra

Special Techniques

27. Enzyme Histochemistry for Tissues - Varinder Uppal, Neelam Bansal, Anuradha Gupta, Opinder Singh and Devendra Pathak

28. Lectin Histochemistry - S. Mayya, K.M. Lasey, K.K. Jayavardhanan, N. Ashok, V.R. Indu, A.R. Sreeranjini, N.S. Sunilkumar and K.B. Suma

29. Sample Preparation for Scanning Electron Microscopy - Balwinder Singh Dhote

30. Transmission Electron Microscopy: A Technique for Subcellular Study of Organs - Pawan Kumar

31. Confocal Microscopy - P. Jagapathi Ramayya and T.S. Chandshekaran Rao

32. Protocol for Immunocytochemical Staining of Stem Cells - T.A. Kannam and Geetha Ramesh

33. Nanotechnology - V. Nagamalayandi

34. Molecular Analysis of Gene by PCR and PCR-RFLP - Lasey, K.M., Mayya, S., Annie, V.R., Ashok, N., Ranjani, C.V., Leena, C., Patki, H. S. and Sariyith, K. P.

35. Commonly used Special Stains in Pathology Laboratory - K.K. Avari and Rakesh Kumar

36. Necropsy Examination Techniques - Rakesh Kumar, K.K. Avari and Naresh Jindal