“Experimental and Evidence based Protocols in Pharmacology” provides a holistic coverage of scientific protocols involved in the pharmacology and toxicological studies. The book serves as a reference to the biomedical researchers working in pharmacology and allied subjects to mitigate the challenges in the routine laboratory procedures.  Book has 22 chapters with evidence based protocols and procedures to conduct the defined experiment. Further, this book provides single reference window with evidence based practical approaches to cater the need of academician and research scholars. The book is designed in a diligent manner to provide majority of protocols viz. Primary cell culture, Mesenchymal stem cells isolation and its application,  DNA fragmentation Assay, oxidative stress evaluation protocols, Cytotoxicity and Genotoxicity Assays, Apoptosis studies, Analytical studies involving HPLC, GC-MS, ICP-OES and experimental pharmacological tool in smooth muscle pharmacological studies etc. to cement the scientific acumen. This book imbibes all the basic and advanced research protocols along with their translational approaches in experiments involved in pharmacology and also provides the insight of example based drug assays to help in bioprospecting of naive molecules.

Further, this book also provide a reference book to the researchers involved in toxicological testing using OECD testing guideline to calculate the lethal dose of any compound in a better manner. This MS also suffice the different methods to calculate the median lethal dose (LD50). The important reproductive toxicity assays to ascertain the toxic potential of any xenobiotics is also incorporated in this MS. Moreover, sophisticated technique like Flow cytometry and other experimental models viz. Direct and Indirect LPS induced acute lung injury model has also well written to provide an insight of the experimentation involving laboratory animals.

Prof. (Dr.) A.K. Srivastava did his graduation and post-graduation in Veterinary Pharmacology from College of Veterinary Science, Mathura. He obtained his doctoral degree in Veterinary Pharmacology and Toxicology from Punjab Agricultural University (PAU), Ludhiana and Post Doctorate Fellowship from Institute of Toxicology, Munchen, Germany. He has also been honoured with Degrees of Doctor of Science (D.Sc. Honoris Causa) from various universities. He served as Chairman, Agricultural Scientists Recruitment Board (ASRB), DARE, Ministry of Agriculture, Government of India, New Delhi, Director cum Vice Chancellor, ICAR-National Dairy Research Institute, Karnal; Dean Faculty of Veterinary Science and Animal Husbandry and Director Resident Instructions and Dean Post graduate Studies at Sher-e-Kashmir University of Agricultural Sciences and Technology (SKUAST-J), Jammu; Head ,Department of Veterinary Pharmacology and Toxicology and Controller of Examinations at PAU, Ludhiana. He Prof. Srivastava is a distinguished Member of National Academy of Sciences. He is feathered with fellow of National Academy of Agricultural Sciences, National Academy of Dairy Sciences and National Academy of Veterinary Sciences. He has been decorated with numerous prestigious awards and honors viz. ICAR Jawaharlal Nehru Award, International NOCIL Award, National Alarsin Awards in 1987-88 and in 1999-2000, ASET Gold Medal, German Academic Exchange Award, Gold Medals of Indian Science Congress Association and National Education Award 2016 etc. He is recipient of Dr. B.D. Garg outstanding Pharmacologist Award, Dr. V. Kurien Memorial Award, Chellappa Memorial Oration Award, Mansinhbhai Memorial Oration Award etc. He was also associated with working group of Planning Commission on “Animal Husbandry and Dairying” for XII Five Year Plan and Mid Term Evaluation of XI Five Year Plan, Government of India as Chairman. He led the DST-India delegation to New Zealand. He also represented India and was Chairman/Member of Indian scientific delegation to Kansas University, USA, Belgium, Dubai, Ethiopia, Sri Lanka, Hong Kong, Nairobi, UK, Australia, New Zealand, Bangkok, Michigan State University, USA etc. He has more than 250 research papers, 10 books and 12 “National Policy Papers” in his credit and has guided more than 25 Ph.D. and post graduate students.

Prof. (Dr.) Atul Prakash did his B.V.Sc.& A.H. from Harbinger of Green Revolution G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand in the year 2004 and M.V.Sc. in Veterinary Pharmacology and Toxicology from N.D University of Agriculture and Technology, Ayodhya in the year 2006. Further he conferred with Doctor of Philosophy in Veterinary Pharmacology from Indian Veterinary Research Institute, Izatnagar, Bareilly in the year 2009. He has more than 15 years of experience in teaching of Pharmacology and Toxicology. He is awarded with several awards viz. Foreign Travel Grant from DBT, Govt. of India and CSIR, Govt. of India etc. He is also awarded with certificate of recognition from various scientific societies. He has guided more than 10 postgraduate research scholars. Currently, he is serving as Professor and Head in the Department of Veterinary Pharmacology and Toxicology, C.V.Sc. & A.H., U.P. Veterinary University (DUVASU), Mathura.

Dr. Amit Shukla, did his B.V.Sc. & A.H. and M.V.Sc. in Veterinary Pharmacology and Toxicology from G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand in the year 2010 and 2012, respectively. He completed his Ph.D. in Veterinary Pharmacology from Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly in the year 2015. He has more than 7 years of teaching experience in the field of Veterinary Pharmacology and Toxicology. He was awarded with several award viz. Best Educationist Award, CARS Project Award from DRDO-INMAS, Ministry of Defence, Government of India, New Delhi, Best poster Award and Best presentation Award etc. Presently, he is working as an Assistant Professor in the Department of  Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura- 281001, Uttar Pradesh, India.

Dr. Soumen Choudhury did his B.V.Sc. & A.H. from CAU, Imphal in the year 2005 and M.V.Sc. in Veterinary Pharmacology and Toxicology from U.P. Veterinary University (DUVASU), Mathura in the year 2007. He completed his Ph.D. in Veterinary Pharmacology from Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly in the year 2014. He has more than 12 years of teaching experience in the field of Veterinary Pharmacology and Toxicology. He was awarded with several award viz. Prof. V.V. Ranade Young Scientist Award by Indian Society of Veterinary Pharmacology & Toxicology (ISVPT) etc. Presently, he is working as an Assistant Professor in the Department of  Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura- 281001, Uttar Pradesh, India.

1    Preparation of Primary Cell Cultures from Different Tissues/Organs

Atul Prakash, Rajesh Mandil, Amit Shukla and A.K. Srivastava

2    Procedures to Evaluate Cytotoxicity in Primary Cell Culture

Atul Prakash, Rajesh Mandil, Amit Shukla and A.K. Srivastava

3    Genotoxicity Assays for Safety Evaluation of Chemicals in Cell Culture

Atul Prakash, Rajesh Mandil and A.K. Srivastava

4    DNA Fragmentation Assay

Rajesh Mandil, Atul Prakash, S. P. Singh and Preeti Singh

5    Single Cell Gel Electrophoresis (SCGE/Comet assay) in Primary Cell Culture

Atul Prakash and Rajesh Mandil

6    Micronuclei Induction Assay in Primary Cell Culture

Atul Prakash and Rajesh Mandil

7    Protocol for TUNEL Assay in Primary Cell Culture

Rajesh Mandil and Atul Prakash

8    Screening of Myometrial Relaxants in Experimental Dysmenorrhoea an in vitro Model

Afroz Jahan, M. K. Lonare, Sanweer Khatoon and G. S. Rao

9    Direct and Indirect LPS Induced Acute Lung Injury Model

Meemansha Sharma, Anshuk Sharma, Thakur Uttam Singh

10  A Laboratory Protocol for Mesenchymal Stem Cell Isolation from Veterinary Important Species

Milindmitra K Lonare, Afroz Jahan, Manjinder Sharma, and Sivaraman Ramanarayanan

11  Detection of Environmental Pollutants and Agrochemicals Using Gas Chromatography and Mass Spectrophotometry

Amit Shukla, Soumen Choudhury, Atul Prakash, Sakshi Tiwari

12  Basics of Flow Cytometric Analysis

Amit Shukla, Mukul Anand, Soumen Choudhury, Sakshi Tiwari

13  Determination of Aflatoxin Using HPLC

Shalini Vaswani, Mukul Anand

14  ICP-OES to Estimate Minerals in Biological Samples

Shalini Vaswani, Mukul Anand

15  Recording of Myometrial Reactivity Using Polyphysiograph

Soumen Choudhury, Amit Shukla, Preeti Singh and Atul Prakash

16  Estimation of Oxidative Stress in Hepatic Tissue

Amit Shukla and Sakshi Tiwari

17  Drug Metabolizing Enzymes Assay

Atul Prakash and Amit Shukla

18  Acetylcholine Esterase (AChE) Assay

Atul Prakash and Amit Shukla

19  Preparation of Hepatocyte Culture for Toxicity Testing of Xenobiotics

S.P. Singh and Atul Prakash

20  Detection of Apoptosis by DNA Gel Electrophoresis

Atul Prakash and Amit Shukla

21  Lethal Dose 50 Calculation Methods

Amit Shukla, Atul Prakash, Soumen Choudhury and Sakshi Tiwari

22  OECD Guidelines for Acute Animal Toxicity Tests

Amit Shukla and Sakshi Tiwari

23  Evaluation of Reproductive Toxicity Potential of Xenobiotics

Atul Prakash and Amit Shukla