Keywords
Restriction endonucleases (Type II / IIS), Recognition sequence & overhangs (sticky vs blunt), DNA ligase (T4) & ligation efficiency, Alkaline phosphatase (vector dephosphorylation), Multiple Cloning Site (MCS), Origin of replication (ori) & copy number, Selectable markers (Amp^R, Kan^R), Reporter systems (lacZ/blue–white), Plasmids, Cosmids, Phagemids, BAC/PAC/YAC vectors, Shuttle & co-integrating vectors, Transposon vectors, Competent cells (CaCl₂ heat-shock), Electroporation, ,Gene cloning & library construction (genomic/cDNA),Probe preparation & labeling (radio/chemiluminescent), Gel electrophoresis (agarose/PAGE), Southern / Northern / Western blotting, PCR & variants (RT-PCR, qPCR, Nested), Gibson Assembly / Golden Gate (Type IIS), ICAR 6th Deans SyllabusRecombinant DNA Technology: Volume 04 : The Question Bank Series - Volume 04
authored by: Archana Rani & Yogendra SinghLength: 152 mm | Breadth: 17.9 mm | Height: 229 mm | Imprint: CONTENT VIBES | Weight: 545 GMS
This book will be available from 25-Sep-2025
Designed for fast revision, this book distills recombinant DNA technology into high-yield notes, flowcharts, and comparison tables exactly the way competitive exams ask it (ICAR PG/PhD, ASRB-NET/ARS Pre, SAU entrances, GATE-BT/XL basics).
Why it helps you score:
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One-page concept maps for each unit: enzymes → vectors → cloning → screening → analysis.
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Must-know tables: Type I/II/IIS REs, recognition sites, overhangs; plasmid/cosmid/BAC/PAC/YAC features; selectable/reporters.
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Workflow cheatsheets: competent cell prep, transformation (heat-shock/electroporation), cloning pipeline, library construction, probe prep.
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Numerical boxes: Tm, insert:vector molar ratios, PCR copy number, gel band size estimation.
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Question bank: unit-wise MCQs (single-best, numerical), Assertion–Reason, Match-the-pairs, figure-based gel/blot questions, with keys & brief explanations.
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Troubleshooting cues: no colonies, smeared gels, non-specific PCR, false positives in blue-white screening.
Archana Rani: Senior Research Fellow (Genetics), Department of Plant Breeding Genetics,, College of Agriculture, Jawahar Lal Nehru Krishi Vishwavidyala, Jabalpur-482004, Madhya Pradesh
Yogendra Singh: Senior Assistant Professor (Biotechnology), Department of Genetics and Plant Breeding, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, Madhya Pradesh
1 Recombinant DNA Technology
2 Restriction Endonucleases: Types and Uses
3 DNA Manipulating Enzymes, DNA Ligases
4 Vectors: Properties of an Ideal Vector, Structure of Vector
5 Cloning Vectors and Expression Vectors
6 Plasmids, Cosmids, Phagemids
7 Bacs, Pacs, Yacs, Transposon Vectors, Shuttle Vectors, Co-Integrating Vectors
8 Competent Cells
9 Gene Isolation and Cloning
10 Genetic Transformation of E. Coli, Gel Electrophoresis
11 Preparation of Probes
12 Southern Blotting; Northern Blotting; Western Blotting
13 PCR and PCR Based Methods in Recombinant DNA Technology