Applications of Genomics and Proteomics provides a comprehensive introduction to modern genomic and proteomic technologies and their applications in biological research, agriculture, health sciences, and biotechnology. The book explains fundamental concepts of plant genomes, comparative genomics, functional genomics, and genome organization with special emphasis on model plants such as Arabidopsis thaliana and important crop species including rice, wheat, tomato, and pigeon pea.
It covers advanced techniques such as RNA interference, site-directed mutagenesis, transposon tagging, transient gene expression, and targeted genome editing. The book also discusses protein structure modelling, proteome analysis, protein identification, and protein–protein interaction studies using modern molecular tools and analytical approaches.
Designed for students, researchers, teachers, and professionals in biotechnology and life sciences, this book combines theoretical concepts with practical applications. It highlights the role of genomics, proteomics, metabolomics, and ionomics in improving agriculture, understanding biological systems, and advancing human health and industrial biotechnology.
Chapter 1. Introduction to Plant Genomes with Focus on Arabidopsis thaliana and Its Role as a Model Organism
Chapter 2. Genomes of Major Crops Including Rice, Tomato, Pigeon Pea, and Wheat
Chapter 3. Comparative Genomics and Genome Organization in Plants
Chapter 4. Functional Genomics Using Mutants and RNA Interference
Chapter 5. Site-Directed Mutagenesis and Transposon Tagging Techniques
Chapter 6. Transient Gene Expression Methods Including Virus-Induced Gene Silencing and FACS-Based Approaches
Chapter 7. Fundamentals of Targeted Genome Editing Technologies and Basic Concepts
Chapter 8. Protein Three-Dimensional Structure Modelling Including Homology Modelling and Crystallography
Chapter 9. Proteome Analysis and Protein Identification Techniques
Chapter 10. Protein–Protein Interaction Studies Using FRET, Yeast Two-Hybrid, Co-Immunoprecipitation, and Bimolecular Fluorescence Complementation
Chapter 11. Applications of Genomics, Proteomics, Metabolomics, and Ionomics in Agriculture, Human Health, Industry, and Metabolic Pathway Analysis