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Molecular Markers and Plant Biotechnology

Tomar, R.S.

  • Country of Origin India
  • ImprintNIPA
  • ISBN9789380235257
  • BindingHB
  • Year of Publishing2010
  • Number Of Pages626 pages, 25cm
  • LanguageEnglish
  • Available Formats

     
    • Hardback

      USD 158.49
      Less 10.00% Discount
      Online Price: USD142.64

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  • About The Author
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  • The book entitled "Molecular Markers and Plant Biotechnology" is an exclusive collection of molecular marker based techniques narrated in 40 s through 578 along with figures makes it essential for biotechnology people. To supplement the practical working the relevant equipments have been described. Laboratory safety rules placed in the beginning is a wise task. Appendices include basic calculations; basic principles in preparation of reagents, abbreviations and glossary show the carefulness while preparing this text. This is an unavoidable text for biotechnology laboratory and class."

  • Rukam S. Tomar: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat. Professor Manoj V. Parakhia:Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat, Professor Sunil Patel: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat. Professor B.A. Golakiya: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat

  • No eChapter available for this eBook
  • 1. Milestones in DNA history and biotechnology. 2. Equipments required in a molecular marker laboratory. 3. Isolation, purification and quantification of genomic DNA from plants. 4. Isolation, purification and quantification of RNA from plants. 5. Electrophoresis. 6. Molecular weight markers for gel electrophoresis. 7. Polycrylamide Gel Electrophoresis (PAGE). 8. Gel electrophoresis of protein. 9. Isoenzyme. 10. Extraction of DNA fragments from Agarose Gel. 11. Why molecular markers? 12. Restriction enzyme digestion of DNA and its Agarose Gel Electrophoresis. 13. Thermocyclers (PCR Machines). 14. Modifications of basic PCR technique. 15. Random amplified polymorphic DNA (RAPD). 16. Restriction Fragment Length Polymorphism (RFLP). 17. Amplified Fragment Length Polymorphism (AFLP). 18. Simple sequence repeats (Microsatellite). 19. Variable number of Tandem repeats (Minisatellite). 20. Inter Simple Sequence Repeat (ISSR). 21. Sequence Characterized Amplified Region (SCAR). 22. Cleaved Amplified Polymorphic Sequence (CAPS). 23. Single-Strand Conformation Polymorphism (SSCP). 24. Retrotransposon-based markers (S-SAP, IRAP, REMAP, RBIP, RGAS, SNP). 25. Silver staining. 26. Basics of marker assisted selection in crop plants. 27. Mapping populations. 28. QTL mapping. 29. Southern blotting. 30. Western blotting. 31. Northern blotting. 32. Eastern blotting. 33. DNA sequencing and designing of primers. 34. Gene transfer to plant. 35. Fluorescent in Situ Hybridization (FISH). 36. Genotypic Barcoding. 37. mtDNA. 38. MicroRNA. 39. Graphical approach to calculate molecular weights of DNA/protein fragments. 40. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.

  • xyd2dx

Browse Subjects

Agricultural Sciences

  • Abiotic and Biotic Stress
  • Agribusiness Management
  • Agriculture Economics
  • Agriculture Engineering
  • Agronomy
  • Biotechnology
  • Crop Management and Improvement
  • Entomology
  • Environment and Climate Change
  • Greenhouse Technology
  • ICT and Extension Education
  • Irrigation and Drainage Engineering
  • Mathematics and Statistics
  • Natural Resources Management
  • Nematology
  • Pest Management
  • Rural Development and Livelihood Security
  • Seed Science and Technology
  • Soil Sciences

Animal Husbandry and Veterinary Sciences

  • Animal Biotechnology
  • Animal Husbandry and Extension
  • Animal Nutrition
  • Animal Production and Reproduction
  • Bacteriology and Microbiology
  • Dairy Sciences
  • Fisheries and Aquaculture
  • Genetics and Breeding
  • Immunology
  • Livestock
  • Pathology and Behaviour
  • Pharmacology and Toxicology
  • Physiology and Anatomy
  • Poultry Science
  • Surgery
  • Veterinary Sciences

Climate Change and Environmental Sciences

  • Biodiversity
  • Climate Change
  • Pollution
  • Renewable Energy
  • Waste Management and Recycling

Food Sciences and Technology

  • Food Science and Technology
  • Food Security and Nutrition
  • Microbiology
  • Processing and Packaging

Geological Sciences

  • Disasters
  • Geology
  • Hydrology
  • Mining
  • Oceanography
  • Remote Sensing and GIS

Horticultural Sciences

  • Agroforestry and Forestry
  • Aromatic and Medicinal Plants
  • Floriculture and Landscaping
  • Fruit Science (Pomology)
  • Plantation and Spices Crops
  • Post Harvest Technology
  • Processing and Preservation
  • Vegetable Science

Plant Sciences

  • Biotechnology and Bioinformatics
  • Plant Breeding and Genetics
  • Plant Pathology
  • Plant Physiology
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