Molecular Markers and Plant Biotechnology

Tomar, R.S.

ImprintNIPA
ISBN 9789380235257
Year2010
Pages626 pages, 25cm
LanguageEnglish

Purchase Options

Printed Price
USD 158.49
Online Purchase Discount 10.00%
Online Purchase Amount USD 142.64

* Delivery and other charges may apply.

The book entitled "Molecular Markers and Plant Biotechnology" is an exclusive collection of molecular marker based techniques narrated in 40 s through 578 along with figures makes it essential for biotechnology people. To supplement the practical working the relevant equipments have been described. Laboratory safety rules placed in the beginning is a wise task. Appendices include basic calculations; basic principles in preparation of reagents, abbreviations and glossary show the carefulness while preparing this text. This is an unavoidable text for biotechnology laboratory and class."
Rukam S. Tomar: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat. Professor Manoj V. Parakhia:Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat, Professor Sunil Patel: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat. Professor B.A. Golakiya: Department of Biochemistry, Junagarh Agricultural University, Junagarh 362 001, Gujarat
1. Milestones in DNA history and biotechnology. 2. Equipments required in a molecular marker laboratory. 3. Isolation, purification and quantification of genomic DNA from plants. 4. Isolation, purification and quantification of RNA from plants. 5. Electrophoresis. 6. Molecular weight markers for gel electrophoresis. 7. Polycrylamide Gel Electrophoresis (PAGE). 8. Gel electrophoresis of protein. 9. Isoenzyme. 10. Extraction of DNA fragments from Agarose Gel. 11. Why molecular markers? 12. Restriction enzyme digestion of DNA and its Agarose Gel Electrophoresis. 13. Thermocyclers (PCR Machines). 14. Modifications of basic PCR technique. 15. Random amplified polymorphic DNA (RAPD). 16. Restriction Fragment Length Polymorphism (RFLP). 17. Amplified Fragment Length Polymorphism (AFLP). 18. Simple sequence repeats (Microsatellite). 19. Variable number of Tandem repeats (Minisatellite). 20. Inter Simple Sequence Repeat (ISSR). 21. Sequence Characterized Amplified Region (SCAR). 22. Cleaved Amplified Polymorphic Sequence (CAPS). 23. Single-Strand Conformation Polymorphism (SSCP). 24. Retrotransposon-based markers (S-SAP, IRAP, REMAP, RBIP, RGAS, SNP). 25. Silver staining. 26. Basics of marker assisted selection in crop plants. 27. Mapping populations. 28. QTL mapping. 29. Southern blotting. 30. Western blotting. 31. Northern blotting. 32. Eastern blotting. 33. DNA sequencing and designing of primers. 34. Gene transfer to plant. 35. Fluorescent in Situ Hybridization (FISH). 36. Genotypic Barcoding. 37. mtDNA. 38. MicroRNA. 39. Graphical approach to calculate molecular weights of DNA/protein fragments. 40. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.
No chapters found..
Send to Author Send to Publisher Recommend to Friend for purchase

Freind's Name:

Friend's E-mail:

Name:

Organization:

Department:

Address:

City:

State:

Zip Code:

Country:

Phone No.:

Fax No.:

E-mail:

Password:

Feedback:

Type the Code Shown: p2rk6y